Callogenesis and Plant Regeneration in Peony (Paeonia × suffruticosa) Using Flower Petal Explants

Peony is a traditional Chinese flower with significant ornamental and medicinal value. However, there are still problems, such as serious browning, difficulties in differentiation, rooting low regeneration efficiency the process of system established, which have hindered development transgenic peony technology. Establishing an efficient considered to be important goal among researchers. Here, we describe protocol for high-frequency callus induction establishment plants using petals explants. Murashige Skoog (MS) medium supplemented 2.0 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D), 1.5 N-(phenylmethyl)-9H-purin-6-amine (6-BA), 0.3 1-naphthylacetic (NAA) was identified best induction, achieving rate up 98.52%. The highest proliferation (234%) achieved on MS 0.2 NAA 3.0 6-BA. differentiation (34.81%) 6-BA 0.5 zeatin (ZT). 23.33% when 1/2 0.1 0.05 3-indolebutyric (IBA). After acclimation, were transferred pots, where they showed robust growth. We also observed surface structures calluses scanning electron microscopy found that characteristics hugely variable different appeared affect bud efficiency. rapid regenerating peonies petal cultures established here will create new opportunities mass reproduction genetic engineering plants.